ABOUT SOME PECULIARITIES OF ARGINASE FLUORESCENCE

Abstract

The fluorescence spectrum of bovine liver arginase excited at 257 nm is similar to that of excited at 291 nm. The alteration of pH values, hydroxylamine treatment and prolonged dialysis leads to the changes in the ratio of fluorescence maximal intensities at mentioned excitation wavelengths (I₂₅₇^max / I₂₉₇^max), which reflects, probably the alteration of microenvironment of aromatic amino acids residues in arginase. The comparative study of the fluorescence spectra of protein solutions at the excitation wavelength 257 and 291 nm can be used as a sensitive method to receive new information about small conformational changes of protein molecules.