INFLUENCE OF IONIC STRENGTH OF SOLUTION ON EtBr AND HOECHST 33258 JOINT BINDING PECULIARITIES WITH DNA

Authors

  • R. A. Karapetyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2010.44.2.045

Keywords:

melting parameters, DNA, Hoechst 33258, ethidium bromide

Abstract

For the first time it is experimentally shown, that at joint interaction of EtBr and Hoechst 33258 (H33258) with DNA depending on their concentration the prevailing influence of one of them on melting parameters of their complexes with biopolymer is observed at ionic strength of solution 0.02 M Na+: at small concentration of ligands (r < 0.05) the influence of EtBr is more expressed. At the rather large concentration influence of H33258 considerably amplifies. At ionic strength of solution 0.002 M Na+ the two ligands influence on DNA melting parameters in the same way.

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Published

2010-05-18

How to Cite

Karapetyan, R. A. 2010. “INFLUENCE OF IONIC STRENGTH OF SOLUTION ON EtBr AND HOECHST 33258 JOINT BINDING PECULIARITIES WITH DNA ”. Proceedings of the YSU B: Chemical and Biological Sciences 44 (2 (222):45-49. https://doi.org/10.46991/PYSU:B/2010.44.2.045.

Issue

Vol. 44 No. 2 (222) (2010)

Section

Biology

License

Copyright (c) 2010 Proceedings of the YSU

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