INVESTIGATION OF THE $RANA~RIDIBUNDA$ TADPOLE’S LIVER ARGINASE TRYPTIC HYDROLYSIS PRODUCTS BY GEL-FILTRATION METHOD

Authors

  • A.S. Shamirian Chair of Biophysics, YSU, Armenia
  • E.Kh. Barseghyan Chair of Biophysics, YSU, Armenia
  • R.H. Grigoryan Chair of Biophysics, YSU, Armenia
  • M.A. Davtyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2013.47.1.028

Keywords:

limited trypsinolysis, Mn$^{2 }$ ions metamorphose, proteolytic inactivation, enzyme pre-incubation

Abstract

In the current study we investigated the Rana ridibunda tadpole’s liver arginase limited trypsinolysis in order to obtain information about the sensitive bonds towards proteases, as well as about the structural and functional interrelations during metamorphose. After 18 h of limited trypsinolysis in the early stages of tadpole development (21–27) appeared relatively massive fragments, the degradation of fragments deepen concomitant to the real metamorphose approach (28–29 stages). Enzyme pre-incubation with Mn2+ leads to stabilization of activity and protects the enzyme from proteolytic inactivation. 

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Published

2013-03-10

How to Cite

Shamirian, A.S., E.Kh. Barseghyan, R.H. Grigoryan, and M.A. Davtyan. 2013. “INVESTIGATION OF THE $RANA~RIDIBUNDA$ TADPOLE’S LIVER ARGINASE TRYPTIC HYDROLYSIS PRODUCTS BY GEL-FILTRATION METHOD”. Proceedings of the YSU B: Chemical and Biological Sciences 47 (1 (230):28-31. https://doi.org/10.46991/PYSU:B/2013.47.1.028.

Issue

Vol. 47 No. 1 (230) (2013)

Section

Biology

License

Copyright (c) 2013 Proceedings of the YSU

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