APPLICATION OF THE VECTOR рЕС-ХК99Е FOR THE CONSTRUCTION OF RECOMBINANT STRAIN-PRODUCERS OF L-ARGININE BASED ON $Brevibacterium$ $flavum$

Authors

  • H.O. Koloyan SPC “Armbiotechnology” SNPO NAS of Republic of Armenia
  • S.V. Avetisyan SPC “Armbiotechnology” SNPO NAS of Republic of Armenia
  • M.H. Paponyan SPC “Armbiotechnology” SNPO NAS of Republic of Armenia
  • H.A. Aganyants SPC “Armbiotechnology” SNPO NAS of Republic of Armenia
  • A.S. Hovsepyan SPC “Armbiotechnology” SNPO NAS of Republic of Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2019.53.2.131

Keywords:

molecular cloning, vector pEC-XK99E, $arg$ genes,$ Brevibacterium$ $ flavum$, strong promoter $Ptrc$, lactose, IPTG

Abstract

Using Escherichia coliCorynebacterium glutamicum shuttle expression vector pEC-XK99E, molecular cloning of the heterologous gene argJ of the thermophilic bacterium Geobacillus stearothermophilus, as well as of homologous genes argG and argH of Corynebacterium glutamicum was carried out in cells of coryneform bacterium Brevibacterium flavum. It was shown that expression of cloned arg genes from the strong promoter Ptrc (its activity is controlled by the protein repressor LacIq) occurred regardless of the presence of transcription inducers lactose or IPTG in the medium.

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Published

2019-07-15

How to Cite

Koloyan, H.O., S.V. Avetisyan, M.H. Paponyan, H.A. Aganyants, and A.S. Hovsepyan. 2019. “APPLICATION OF THE VECTOR рЕС-ХК99Е FOR THE CONSTRUCTION OF RECOMBINANT STRAIN-PRODUCERS OF L-ARGININE BASED ON $Brevibacterium$ $flavum$”. Proceedings of the YSU B: Chemical and Biological Sciences 53 (2 (249):131-37. https://doi.org/10.46991/PYSU:B/2019.53.2.131.

Issue

Section

Biology