BINDING PECULIARITIES OF POLY(rA)-POLY(rU) WITH MINOR GROOVE LIGAND HOECHST 33258

Authors

  • Marine A. Parsadanyan Chair of Biophysics, YSU, Armenia
  • Mariam A. Shahinyan Chair of Biophysics, YSU, Armenia
  • Zvart H. Movsisyan Chair of Biophysics, YSU, Armenia
  • Ara P. Antonyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2021.55.2.144

Keywords:

synthetic polynucleotide, intercalator, minor groove binder, absorption spectra, fluorescence spectra

Abstract

Study on the interaction of DNA-specific ligands – classical intercalator acridine orange (AO) and groove binding compound Hoechst 33258 (H33258) with poly(rA)-poly(rU), being a model for double-stranded (ds-) RNA, has been carried out. The absorption and fluorescence spectra of the complexes of these ligands with ds-polynucleotide were obtained. It was revealed that the optic and fluorescent characteristics of the complexes of both ligands with ds-RNA are similar with those at the complex-formation with DNA.

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Published

2021-08-30

How to Cite

Parsadanyan, Marine A., Mariam A. Shahinyan, Zvart H. Movsisyan, and Ara P. Antonyan. 2021. “BINDING PECULIARITIES OF POLY(rA)-POLY(rU) WITH MINOR GROOVE LIGAND HOECHST 33258”. Proceedings of the YSU B: Chemical and Biological Sciences 55 (2 (255):144-50. https://doi.org/10.46991/PYSU:B/2021.55.2.144.

Issue

Vol. 55 No. 2 (255) (2021)

Section

Biology

License

Copyright (c) 2021 Proceedings of the YSU

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