ANTICANCER EFFECT OF AgTOEPyP4 PORPHYRIN

Abstract

The silver-containing porphyrin Ag-meso-tetra(4-N-hydroxyethylpyridyl)porphyrin (AgTOEPyP4) was investigated as a potential anticancer drug using spectrophotometric, calorimetric and electrophoretic measurements. The studies were conducted on healthy, cancer-induced, and treated by AgTOEPyP4 white mice. DNA was isolated from the liver and tumor tissues of mice. Melting curves were studied in the presence of various stoichiometric concentrations of Mn²⁺ ions. The character of the DNA–Mn²⁺ interaction in the tumor differs from that in the norm due to defects in the secondary structure of tumor DNA, which is expressed in the DNA melting characteristics of DNA. It was revealed that the melting parameters, enthalpy, and electrophoretic mobility of DNA isolated from the tumor-bearing mice tissues differed from healthy DNA. It was shown that all studied parameters of the DNA isolated from the liver and tumor treated by AgTOEPyP4 mice approached the norm. The obtained results revealed that AgTOEPyP4 porphyrin has a pronounced therapeutic effect against sarcoma S180 and requires further investigation as an anticancer drug.