ROLE OF GLYCINE-BETAINE IN THE GROWTH AND HYDROGENASES ACTIVITY OF $RALSTONIA~EUTROPHA$ H16
DOI:
https://doi.org/10.46991/PYSU:B/2023.57.2.154Keywords:
glycine-betaine, Ralstonia eutropha H16, [NiFe]-hydrogenasesAbstract
Glycine-betaine (GB) is an abundant organic material in soil, and the chemolithoautotrophic soil bacterium R. eutropha H16, one of the best bio-technological research models, may involve it in metabolism. The effect of different concentrations of GB (7–300 µmol/mL) on bacterial growth parameters and H2-oxidizing activity of hydrogenases (Hyd) was studied. Stimulation of bacterial growth was registered at GB low concentrations, however, high concentrations have a partial inhibitory effect, in contrast to control (Fructose-Nitrogen medium). Upon GB supplementation, the min and max H2-oxidizing Hyd activity of R. eutropha whole cells were 3.4±0.01 U (g CDW)−1 and 16.4±0.01 U (g CDW)−1, respectively at concentrations of 7 µmol/mL and 300 µmol/mL, while it is absent in the control. These results might be used to develop new approaches to produce oxygen-tolerant Hyd.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Proceedings of the YSU
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.