DETERMINATION OF DNA MELTING CURVES AND DIFFERENTIAL MELTING CURVES FROM CALORIMETRIC DATA
DOI:
https://doi.org/10.46991/PYSU:A/2013.47.1.059Keywords:
differential scanning calorimetry, high-resolution melting profiles, DNA plasmids, Calf Thymus DNAAbstract
For different types of DNA sequences it is demonstrated that a calorimetric melting curve calculated as a temperature dependence of a relative heat absorbance caused by DNA helix-coil transition is very close to the “real” melting curve that is the temperature dependence of the fraction of melted base pairs. There is the same closeness for the calorimetric differential melting curves (DMC) and the values of melting temperatures and temperature melting ranges. A simple procedure of recalculation of a calorimetric DMC into a real DMC is proposed.
Downloads
Published
2013-04-10
How to Cite
Grigoryan, I. (2013). DETERMINATION OF DNA MELTING CURVES AND DIFFERENTIAL MELTING CURVES FROM CALORIMETRIC DATA. Proceedings of the YSU A: Physical and Mathematical Sciences, 47(1 (230), 59–64. https://doi.org/10.46991/PYSU:A/2013.47.1.059
Issue
Section
Physics
License
Copyright (c) 2013 Proceedings of the YSU
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.