DETERMINATION OF MELTING TEMPERATURE FOR MULTI-PEAK DIFFERENTIAL MELTING CURVES OF DNA

Authors

  • D.Yu. Lando Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
  • A.S. Fridman Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
  • L.E. Grigoryan Chair of Molecular Physics, YSU, Armenia
  • E.N. Galyuk Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus

DOI:

https://doi.org/10.46991/PYSU:A/2013.47.3.057

Keywords:

differential scanning calorimetry, high-resolution melting profiles, DNA plasmids, Calf Thymus DNA

Abstract

This study was carried out because of a widely spread mistaken viewpoint that melting temperature $(T_m)$ cannot be used as a reasonable parameter in the case of multi-peak differential melting curves (DMC) of DNA. However, there were no theoretical or experimental evidences or disclaimers of this viewpoint. In this work such study has been carried out. We have tested various definitions of $T_m$. Indeed, some of them give unreasonable dependences of Tm on relative concentration of modifications $(r_b)$. At same time, the average temperature of the helix-coil transition calculated as the integral over the temperature of the product of DMC and temperature gives reasonable smooth dependences $T_m(r_b)$. The same definition is the best for the dependence of $T_m$ on the average GC-content.

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Published

2013-11-20

How to Cite

Lando, D., Fridman, A., Grigoryan, L., & Galyuk, E. (2013). DETERMINATION OF MELTING TEMPERATURE FOR MULTI-PEAK DIFFERENTIAL MELTING CURVES OF DNA. Proceedings of the YSU A: Physical and Mathematical Sciences, 47(3 (232), 57–63. https://doi.org/10.46991/PYSU:A/2013.47.3.057

Issue

Section

Physics