DETERMINATION OF MELTING TEMPERATURE FOR MULTI-PEAK DIFFERENTIAL MELTING CURVES OF DNA
DOI:
https://doi.org/10.46991/PYSU:A/2013.47.3.057Keywords:
differential scanning calorimetry, high-resolution melting profiles, DNA plasmids, Calf Thymus DNAAbstract
This study was carried out because of a widely spread mistaken viewpoint that melting temperature $(T_m)$ cannot be used as a reasonable parameter in the case of multi-peak differential melting curves (DMC) of DNA. However, there were no theoretical or experimental evidences or disclaimers of this viewpoint. In this work such study has been carried out. We have tested various definitions of $T_m$. Indeed, some of them give unreasonable dependences of Tm on relative concentration of modifications $(r_b)$. At same time, the average temperature of the helix-coil transition calculated as the integral over the temperature of the product of DMC and temperature gives reasonable smooth dependences $T_m(r_b)$. The same definition is the best for the dependence of $T_m$ on the average GC-content.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2013 Proceedings of the YSU
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.