EFFECT OF LIGAND BINDING ON FUNCTIONALITY OF DNA FIELD-EFFECT TRANSISTOR

Authors

  • Ye.Sh. Mamasakhlisov Chair of Molecular Physics, YSU, Armenia
  • A.P. Antonyan Chair of Biophysics, YSU, Armenia
  • A.A. Hakobyan Institute of Radiophysics and Electronics, Ashtarak, Armenia

DOI:

https://doi.org/10.46991/PYSU:A/2017.51.1.066

Keywords:

ligand binding, thermostability, hybridization, DNA-chip

Abstract

Motivated by the prospects of developing DNA field-effect transistors, as tools for a variety of application fields such as medical diagnostics, environmental pollutants monitoring, biological weapons defense, and taking into account that the efficiency of DNA-sensors depends on the precise prediction of experimental parameters responsible for thermostability of nucleic acids duplexes and specific times of formation of DNA duplexes, we analyze the factors influencing both the thermodynamics of hybridization and the stability of DNA–DNA and DNA–RNA duplexes. In this work the case of competition-free DNA hybridization is analyzed. It is shown how the intercalating ligands effectively increase the binding constant for the target sequences and thus affects the sensitivity of the DNA-chip.

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Published

2017-03-20

How to Cite

Mamasakhlisov, Y., Antonyan, A., & Hakobyan, A. (2017). EFFECT OF LIGAND BINDING ON FUNCTIONALITY OF DNA FIELD-EFFECT TRANSISTOR. Proceedings of the YSU A: Physical and Mathematical Sciences, 51(1 (242), 66–70. https://doi.org/10.46991/PYSU:A/2017.51.1.066

Issue

Section

Physics