STABILITY AND CONDUCTIVITY OF BILAYER LIPID MEMBRANE IN PRESENCE $\mathrm{Al_2O_3}$ NANOPARTICLES

Tsovinar M. Jomardyan; Gayane V. Ananyan; Valery B. Arakelyan

doi:10.46991/PYSU:A/2023.57.1.023

Authors

Tsovinar M. Jomardyan Chair of Molecular Physics, YSU, Armenia
Gayane V. Ananyan Interfaculty Scientific-Research Laboratory of Structural Biophysics, YSU, Armenia
Valery B. Arakelyan Chair of Molecular Physics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:A/2023.57.1.023

Keywords:

aluminum oxide nanoparticles, stability and conductivity of BLM

Abstract

The effect of aluminum oxide nanoparticles (Al₂O₃) on the stability and conductivity of BLM (the bilayer lipid membrane) in solution was studied. It has been shown that Al₂O₃nanoparticles increase the stability of BLM in an electric field, and BLM becomes more stable with increasing their concentration. The experimental data are analyzed in terms of the well-known theory of BLM stability, which is based on the concept of spontaneous formation of microscopic pores on the BLM, the development of which leads to the loss of BLM stability. It is shown that aluminum oxide nanoparticles increase the value of the coefficient of linear tension of the pore edge, and with an increase in the concentration of nanoparticles, the linear tension also increases. It has been shown that the presence of nanoparticles in the solution leads to a decrease in BLM conductivity.

References

Syrma E.I. Physical Properties of Nanoparticles and Their Biological Effects. Integrative Anthropology 1 (2013), 30-33 (in Russian).

Andreev G.B., Minashkin V.M., et al. Materials Produced by Nanotechnologies: Potential Risk in Obtaining and Using. Ros. Chem. and (J. Russian Chemical Society Named After D.I. Mendeleev) LII (2008), 32-38 (in Russian).

Bondarenko O., Juganson K., Ivask A. Toxicity of Ag, CuO and ZnO Nanoparticles to Selected Environmentally Relevant Test Organisms and Mammalian Cells in vitro: A Critical Review. Archives of Toxicology 87 (2013), 1181-1200. https://doi.org/10.1007/s00204-013-1079-4

Böhme S., Stärk H., et al. Quantification of Al2O3 Nanoparticles in Human Cell Lines Applying Inductively Coupled Plasma Mass Spectrometry (neb-ICP-MS, LA-ICP-MS) and Flow Cytometry-based Methods. J. Nanopart. Res. 16 (2014). https://doi.org/10.1007/s11051-014-2592-y

Sarapultsev A.P., Rempel C.V., et al. Interaction of Nanoparticles with Biological Objects. Bulletin of the Ural Medical Academic Science 3 (2016), 97-110 (in Russian).

Ivkov V.G., Berestovsky G.N. Lipid Bilayer of Biological Membranes. Moscow, Nauka (1982), 224 (in Russian).

Zaitseva N.V., Zemlyanova M.A., et al. Evaluation of Toxicity and Potential Hazard of Aluminum Oxide Nanoparticles for Human Health. Human Ecology 5 (2018), 9-15 (in Russian).

Mueller P., Rudin D.O., et al. Methods for the Formation of Single Bimolecular Lipid Membranes in Aqueous Solution. J. Phys. Chem. 67 (1963), 534-535.

Abidor I.G., Arakelyan V.B., et al. Electric Breakdown of Bilayer Lipid Membranes I. The Main Experimental Facts and Their Qualitative Discussion. Bioelectrochemistry and Bioenergetics 6 (1979), 37-52.

Melikyan G.B., Matinyan N.S., Arakelian V.B. The Influence of Gangliosides on the Hydrophilic Pore Edge Line Tension and Monolayer Fusion of Lipid Membranes. Biochim. Biophys. Acta 1030 (1990), 11-15.

Torosyan A.L., Arakelyan V.B. Influence of H2TOEtPyP4 Porphyrin on the Stability and Conductivity of Bilayer Lipid Membranes. Eur. Biophys. J. 44} (2015), 745-750. https://doi.org/10.1007/s00249-015-1074-1

Ivkov V.G., Berestovsky G.N. Dynamic Structure of the Lipid Bilayer. Moscow, Nauka (1981) (in Russian).

Chernomordik L.V., Kozlov M.M., et al. Shape of Lipid Molecules and Monolayer Membrane Fusion. Biological Membranes 1 (1984), 411-427 (in Russian).

Sokolov A.V. Study of the Effects of Products of the Visual Cycle on Bilayer Lipid Membranes. Ph.D. Thesis. Moscow (2009), 23 (in Russian).

Chizmadzhev Yu.A., Chernomordik L.V., et al. Electrical Breakdown of Bilayer Lipid Membranes. In: Results of Science and Technology. Biophysics of Membranes 2 (1982), 161-266 (in Russian).

Markin V.S., Kozlov M.M. Pore Statistics in Bilayer Lipid Membranes. Biological Membranes 2 (1985), 205-223 (in Russian).

Ghosh S., Mashayekhi H., et al. Colloidal Behavior of Aluminum Oxide Nanoparticles as Affected by pH and Natural Organic Matter. Langmuir 24 (2008), 12385-12391. https://doi.org/10.1021/la802015f

Mui J., Ngo J., Kim B. Aggregation and Colloidal Stability of Commercially Available Al2O3 Nanoparticles in Aqueous Environments. Nanomaterials 6 (2016), 90-104. https://doi.org/10.3390/nano6050090

Pedersen U., Leidy C., et al. The Effect of Calcium on the Properties of Charged Phospholipid Bilayers. Biochimica et Biophysica Acta (BBA). Biomembranes 1758 (2006), 573-582. https://doi.org/10.1016/j.bbamem.2006.03.035

Shevchenko E.V., Antonov V.F. Influence of Divalent Ions on the Physical Properties of Bilayer Lipid Membranes from Zviterrionic and Acidic Phospholipids. Siberian Medical Journal (Irkutsk) 3 (1995), 5-8 (in Russian).