ESTIMATION OF CHROMOSOME X DAMAGE INDUCED WITH MITOMYCIN C IN HUMAN LEUKOCYTES BY MN-FISH METHOD

Authors

  • G. G. Hovhannisyan Chair of Genetics and Cytology, YSU, Armenia
  • A. I. Avagyan Chair of Genetics and Cytology, YSU, Armenia
  • G. I. Tadevosyan Chair of Genetics and Cytology, YSU, Armenia
  • R. M. Aroutiounian Chair of Genetics and Cytology, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2009.43.3.058

Keywords:

fluorescent in situ hybridization, micronuclei, chromosome, leukocytes, male and female

Abstract

Combination of micronuclei test with fluorescent in situ hybridization (FISH) with application of whole chromosome probes was applied to compare chromosome X damage in leukocytes of male and female. Mitomycin C was selected as typical clastogene agent. 916 micronuclei in cells of both genders were studied. The data obtained showed that frequency of distribution of X-chromosome in women's and men’s micronuclei was 4.25 and 1.05% consequently. Higher probability of damage of female inactive X-chromosome in micronuclei compared with its functioning homolog is demonstrated and discussed.

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Published

2009-10-22

How to Cite

Hovhannisyan, G. G., A. I. Avagyan, G. I. Tadevosyan, and R. M. Aroutiounian. 2009. “ESTIMATION OF CHROMOSOME X DAMAGE INDUCED WITH MITOMYCIN C IN HUMAN LEUKOCYTES BY MN-FISH METHOD ”. Proceedings of the YSU B: Chemical and Biological Sciences 43 (3 (220):58-61. https://doi.org/10.46991/PYSU:B/2009.43.3.058.

Issue

Vol. 43 No. 3 (220) (2009)

Section

Short Communications

License

Copyright (c) 2009 Proceedings of the YSU

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.