INFLUENCE OF DIMETHYLSULFOXIDE ON UREA-BOVINE SERUM ALBUMIN INTERACTIONS
DOI:
https://doi.org/10.46991/PYSU:B/2012.46.2.008Keywords:
dimethylsulfoxide, urea bovine serum albumin, thermal and chemical denaturation, reversible multistep mechanismAbstract
Using classical physicochemical (calorimetry, electroconductivity, pH-metry) and spectroscopic (IR, UV/vis) methods the effect of dimethylsulfoxide (DMSO) on urea bovine serum albumin (BSA) interactions have been studied. First of all the structural peculiarities of urea aqueous solutions in the presence of DMSO and then in these solutions the thermal and chemical denaturation of BSA has been determined. It has been established that in DMSO containing solutions (up to 10%, v/v) urea solvate shell structure changes by inclusion of DMSO molecules. At mentioned conditions BSA thermal and chemical denaturation proceeds according to reversible multistep mechanism. After denaturation some structural unities of BSA ternary structure remain.
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