INVESTIGATION OF ACIDIC DENATURATION OF ETHIDIUM BROMIDE COMPLEXES WITH DNA

Authors

  • Zh.H. Mukhaelyan Chair of Biophysics, YSU, Armenia
  • A.P. Antonyan Chair of Biophysics, YSU, Armenia
  • M.V. Minasyants Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2014.48.2.048

Keywords:

DNA, EtBr, thermo-induced denaturation, acidic denaturation, transition point, transition interval width

Abstract

In the present work the temperature-induced and the acidic denaturation of ethidium bromide (EtBr) complexes with DNA have been carried out. The curves of helix-coil transition of both DNA and its complexes with EtBr in 0 ≤ r ≤ 0.5 (r = [ligand] / [DNA]) were obtained. It has been shown that with enhancement of EtBr concentration the transition point is shifted to the “high values” for both factors invoking this transition. It was also revealed that EtBr interacts with DNA by several modes being in non protonated and

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Published

2014-07-01

How to Cite

Mukhaelyan, Zh.H., A.P. Antonyan, and M.V. Minasyants. 2014. “INVESTIGATION OF ACIDIC DENATURATION OF ETHIDIUM BROMIDE COMPLEXES WITH DNA”. Proceedings of the YSU B: Chemical and Biological Sciences 48 (2 (234):48-53. https://doi.org/10.46991/PYSU:B/2014.48.2.048.

Issue

Vol. 48 No. 2 (234) (2014)

Section

Biology

License

Copyright (c) 2014 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.