ETHIDIUM BROMIDE BINDING TO POLY(G): DEPENDENCE ON Na$^+$-CONCENTRATION

Authors

  • A.P. Antonyan Chair of Biophysics, YSU, Armenia
  • M.V. Minasyants Chair of Biophysics, YSU, Armenia
  • P.O. Vardevanyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2015.49.2.034

Keywords:

EtBr–[poly(G)]$_4$ complexes, ionic strength of solution, semiintercalation

Abstract

In the current work the investigation of interaction between polyguanylic acid and ethidium bromide (EtBr) at different ionic strengths and at three different temperatures has been carried out. Based on absorption spectra of formed complexes, adsorption isotherms were constructed and binding constants were determined. Thermodynamic parameters of this interaction were determined as well. Obtained data show that in four-stranded structure, the “classical” intercalation becomes impossible and the only possible binding mode remains semiintercalation. It was also shown that thermodynamic parameters of interaction by semi-intercalation mode do not strongly depend on the ionic strength of solution.

Downloads

Published

2015-07-10

How to Cite

Antonyan, A.P., M.V. Minasyants, and P.O. Vardevanyan. 2015. “ETHIDIUM BROMIDE BINDING TO POLY(G): DEPENDENCE ON Na$^+$-CONCENTRATION”. Proceedings of the YSU B: Chemical and Biological Sciences 49 (2 (237):34-39. https://doi.org/10.46991/PYSU:B/2015.49.2.034.

Issue

Vol. 49 No. 2 (237) (2015)

Section

Biology

License

Copyright (c) 2015 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.