HOECHST 33258 COMPOUND INTERACTION WITH DIFFERENT GC-CONTENT DNA

Authors

  • A.P. Antonyan Chair of Biophysics, YSU, Armenia
  • M.A. Parsadanyan Chair of Biophysics, YSU, Armenia
  • M.A. Torosyan Chair of Physics and Electrotechnics NUACA, Armenia
  • A.T. Karapetyan Chair of Physics and Electrotechnics NUACA, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2019.53.2.107

Keywords:

GC-content of DNA, Hoechst 33258, minor groove, AT-specificity, melting parameters

Abstract

The interaction of Hoechst 33258 (H33258) compound with different GC-content DNA has been studied using the melting method. The obtained data show that at low ionic strengths of the solution (~0.002 M) H33258 does not display specificity for DNA AT sequences. Moreover, the effect was revealed not to depend on DNA nucleotide sequence. It has been shown that the increase in the ionic strength by an order of magnitude results in the development of a pronounced specificity of H33258 for DNA AT-sequences. H33258 was also found to exhibit a higher affinity for DNA having a block structure.

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Published

2019-07-15

How to Cite

Antonyan, A.P., M.A. Parsadanyan, M.A. Torosyan, and A.T. Karapetyan. 2019. “HOECHST 33258 COMPOUND INTERACTION WITH DIFFERENT GC-CONTENT DNA”. Proceedings of the YSU B: Chemical and Biological Sciences 53 (2 (249):107-12. https://doi.org/10.46991/PYSU:B/2019.53.2.107.

Issue

Vol. 53 No. 2 (249) (2019)

Section

Biology

License

Copyright (c) 2019 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.