INTERACTION OF POLY(I) WITH ETHIDIUM BROMIDE

Authors

  • S.N. Hakobyan Department of Mechanical Engineering SEUA, Armenia
  • R.S. Ghazaryan Chair of Molecular Physics, YSU, Armenia
  • M.A. Shahinyan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2017.51.1.038

Keywords:

poly(I), ethidium bromide, complex-formation, absorption and fluorescence spectra

Abstract

The interaction of ethidium bromide (EtBr) with polyinosine (poly(I)) has been studied by absorption and fluorescence spectroscopy methods. Our investigations have shown that titration experiments within raising of poly(I) concentration the maximum of absorption in visible region decreases and is shifted toward longer wavelength region. According to the obtained results it is assumed that poly(I) in physiological conditions exists both in single- and double-stranded helices formed contemporaneously. The studies are relevant to understand the polyinosine structure in aqueous-salt solution.

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Published

2017-03-30

How to Cite

Hakobyan, S.N., R.S. Ghazaryan, and M.A. Shahinyan. 2017. “INTERACTION OF POLY(I) WITH ETHIDIUM BROMIDE”. Proceedings of the YSU B: Chemical and Biological Sciences 51 (1 (242):38-43. https://doi.org/10.46991/PYSU:B/2017.51.1.038.

Issue

Vol. 51 No. 1 (242) (2017)

Section

Biology

License

Copyright (c) 2017 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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