CHEMICAL COMPOSITION AND ANTIMICROBIAL POTENTIAL OF ESSENTIAL OIL OF $ARTEMISIA~DRACUNCULUS$ L., CULTIVATED AT HIGH ALTITUDE ARMENIAN LANDSCAPE
DOI:
https://doi.org/10.46991/PYSU:B/2018.52.2.116Keywords:
$Artemisia~dragunculus$, essential oil, estragol, antimicrobial activityAbstract
The gas chromatography-mass spectrometry setup was used to reveal the chemical composition of essential oil (EO) of A. dracunculus cultivated at the altitude of 1700–1800 m above sea level (Aragyugh, Armenia) and harvested during the blossoming period (July, 2015). Estragol (methyl chavicol) in EO reached 84.9%. The other components were linalool (5.09%), trans-beta-ocimen (4.00%), limonene (1.63%), (Z,E)-alloocimene (2.29%), 3-caren (0.81%) and beta-ocimen (0.61%). Antimicrobial activity of EO from A. dracunculus L. was determined by agar diffusion method. Gram-positive, gram-negative and ampicillinresistant bacteria, as well as some yeasts were tested. The concentrations of EO from 1.5625 to 150 mg/mL were used. The minimal inhibitory concentration (MIC) of A. dracunculus EO was 6.25 mg/mL against St. aureus and B. subtilis. MIC value against E. coli VKPM-M17 was 50 mg/mL, but P. aeruginosa was less susceptible to EO components and MIC value reached 150 mg/mL. The antibiotic resistant E. coli dhpα-pUCl8 strain possessed high sensitivity against the ЕО with 6.25 mg/mL MIC value. The action of ЕО was bactericidal. Tested yeasts were more susceptible against oil component, MIC=1.56 mg/mL. The obtained results show that A. dracunculus EO can be useful for cosmetics, medicine and food as antimicrobial natural agent.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 Proceedings of the YSU
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.