STUDY OF INTERACTION OF HOECHST 33258 WITH DNA AND HUMAN SERUM ALBUMIN UNDER THE INFLUENCE OF MILLIMETER RANGE ELECTROMAGNETIC WAVES

Authors

  • Poghos H. Vardevanyan Chair of Biophysics, YSU, Armenia
  • Mariam A. Shahinyan Chair of Biophysics, YSU, Armenia
  • Anna V. Vardanyan Chair of Biophysics, YSU, Armenia
  • Svetlana V. Grigoryan Chair of Biophysics, YSU, Armenia

DOI:

https://doi.org/10.46991/PYSU:B/2021.55.2.136

Keywords:

millimeter range electromagnetic waves, macromolecule, ligand, absorption spectra, fluorescence spectra, binding constant, Stern–Volmer constant

Abstract

In this work the effect of millimeter range electromagnetic waves (MM EMW) with the frequency 64.5 GHz on the complexes of Hoechst 33258 (H33258) with DNA and human serum albumin (HSA) has been studied by the methods of absorption and fluorescence spectroscopies. It was shown that the irradiation results in weakening of H33258 interaction with both macromolecules, which is connected with the fact that the frequency 64.5 GHz, being resonant for water, leads to the structurizing of water component around DNA and HSA, due to which the binding becomes weaker. This conclusion is based on the values of both binding constants and Stern–Volmer constants.

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Published

2021-08-30

How to Cite

Vardevanyan, Poghos H., Mariam A. Shahinyan, Anna V. Vardanyan, and Svetlana V. Grigoryan. 2021. “STUDY OF INTERACTION OF HOECHST 33258 WITH DNA AND HUMAN SERUM ALBUMIN UNDER THE INFLUENCE OF MILLIMETER RANGE ELECTROMAGNETIC WAVES”. Proceedings of the YSU B: Chemical and Biological Sciences 55 (2 (255):136-43. https://doi.org/10.46991/PYSU:B/2021.55.2.136.

Issue

Vol. 55 No. 2 (255) (2021)

Section

Biology

License

Copyright (c) 2021 Proceedings of the YSU

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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