ABSORPTION AND FLUORESCENCE SPECTRA OF THE COMPLEXES OF METHYLENE BLUE AND ACRIDINE ORANGE WITH POLY(rA)-POLY(rU)
DOI:
https://doi.org/10.46991/PYSU:B/2021.55.2.151Keywords:
synthetic polynucleotide, methylene blue, acridine orange, absorption spectrum, fluorescence spectrumAbstract
The interaction of methylene blue (MB) and acridine orange (AO) with poly(rA)-poly(rU) was studied using absorption and fluorescence spectroscopy methods. The absorption and fluorescence spectra of complexes of these ligands with a polynucleotide were obtained, similar to those of complexes of these ligands with double-stranded (ds-) DNA. It was revealed that the isosbestic point in the spectra of the AO-poly(rA)-poly(rU) and MB-poly(rA)-poly(rU) complexes is not formed, although the binding of AO and MB with ds-RNA by intercalation mode is not excluded.
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Published
2021-08-30
How to Cite
Shahinyan, Mariam A., Zvart H. Movsisyan, Gohar G. Hovhannisyan, and Poghos H. Vardevanyan. 2021. “ABSORPTION AND FLUORESCENCE SPECTRA OF THE COMPLEXES OF METHYLENE BLUE AND ACRIDINE ORANGE WITH POLY(rA)-POLY(rU)”. Proceedings of the YSU B: Chemical and Biological Sciences 55 (2 (255):151-57. https://doi.org/10.46991/PYSU:B/2021.55.2.151.
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Biology
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