THE INTERACTION OF Ag-CONTAINING PORPHYRINS WITH DNA AT HIGH IONIC STRENGTH
DOI:
https://doi.org/10.46991/PYSU:A/2009.43.2.055Keywords:
DNA, porphyrin, ionic strengthAbstract
UV-VIS spectrophotometry and circular dichroism (CD) methods were applied to study the binding of DNA with three novel Ag-containing porphyrins meso-tetra(4N-allylpyridyl) (AgTAlPyP4), meso-tetra(4N-butylpyridyl) (AgTButPyP4) and meso-tetra(4N-oxyethylpyridyl) (AgTOEtPyP4) at high ionic strength. The binding constant Kb and stoichiometry n were determined from binding isotherms for each ligand–DNA complex. The Gibbs free energy, enthalpy and entropy of binding also were calculated using the values of Kb . Obtained results show that these porphyrins associate with the duplex DNA via outside binding mode.
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